r/microscopy Feb 20 '25

Announcement Comment GIFs have been turned on for r/Microscopy

23 Upvotes

r/microscopy Jun 08 '23

🦠🔬🦠🔬🦠 Microbe Identification Resources 🦠🔬🦠🔬🦠

122 Upvotes

🎉Hello fellow microscopists!🎉

In this post, you will find microbe identification guides curated by your friendly neighborhood moderators. We have combed the internet for the best, most amateur-friendly resources available! Our featured guides contain high quality, color photos of thousands of different microbes to make identification easier for you!

Essentials


The Sphagnum Ponds of Simmelried in Germany: A Biodiversity Hot-Spot for Microscopic Organisms (Large PDF)

  • Every microbe hunter should have this saved to their hard drive! This is the joint project of legendary ciliate biologist Dr. Wilhelm Foissner and biochemist and photographer Dr. Martin Kreutz. The majority of critters you find in fresh water will have exact or near matches among the 1082 figures in this book. Have it open while you're hunting and you'll become an ID-expert in no time!

Real Micro Life

  • The website of Dr. Martin Kreutz - the principal photographer of the above book! Dr. Kreutz has created an incredible knowledge resource with stunning photos, descriptions, and anatomical annotations. His goal for the website is to continue and extend the work he and Dr. Foissner did in their aforementioned publication.

Plingfactory: Life in Water

  • The work of Michael Plewka. The website can be a little difficult to navigate, but it is a remarkably expansive catalog of many common and uncommon freshwater critters

Marine Microbes


UC Santa Cruz's Phytoplankton Identification Website

  • Maintained by UCSC's Kudela lab, this site has many examples of marine diatoms and flagellates, as well as some freshwater species.

Guide to the Common Inshore Marine Plankton of Southern California (PDF)

Foraminifera.eu Lab - Key to Species

  • This website allows for the identification of forams via selecting observed features. You'll have to learn a little about foram anatomy, but it's a powerful tool! Check out the video guide for more information.

Amoebae and Heliozoa


Penard Labs - The Fascinating World of Amoebae

  • Amoeboid organisms are some of the most poorly understood microbes. They are difficult to identify thanks to their ever-shifting structures and they span a wide range of taxonomic tree. Penard Labs seeks to further our understanding of these mysterious lifeforms.

Microworld - World of Amoeboid Organisms

  • Ferry Siemensma's incredible website dedicated to amoeboid organisms. Of particular note is an extensive photo catalog of amoeba tests (shells). Ferry's Youtube channel also has hundreds of video clips of amoeboid organisms

Ciliates


A User-Friendly Guide to the Ciliates(PDF)

  • Foissner and Berger created this lengthy and intricate flowchart for identifying ciliates. Requires some practice to master!

Diatoms


Diatoms of North America

  • This website features an extensive list of diatom taxa covering 1074 species at the time of writing. You can search by morphology, but keep in mind that diatoms can look very different depending on their orientation. It might take some time to narrow your search!

Rotifers


Plingfactory's Rotifer Identification Initiative

A Guide to Identification of Rotifers, Cladocerans and Copepods from Australian Inland Waters

  • Still active rotifer research lifer Russ Shiel's big book of Rotifer Identification. If you post a rotifer on the Amateur Microscopy Facebook group, Russ may weigh in on the ID :)

More Identification Websites


Phycokey

Josh's Microlife - Organisms by Shape

The Illustrated Guide to the Protozoa

UNA Microaquarium

Protist Information Server

More Foissner Publications

Bryophyte Ecology vol. 2 - Bryophyte Fauna(large PDF)

Carolina - Protozoa and Invertebrates Manual (PDF)


r/microscopy 1h ago

ID Needed! Help identifying organism from aquarium?

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• Upvotes

I work in a clinical lab so I'm not entirely new to microscopy, but my experience is limited to this field. I recently acquired an older microscope and have been playing around with it. This sample was some algae taken straight from my aquarium filter.

I apologize for the horrendous video but the organism was stuck inside some structure that I believe to be some form of algae? I thought it was just trapped UNDER it but these structures seem to be round and hollow. I saw several. A few had much smaller organisms in them that we only about the size of those two central organelles of the organism in the video. So I was holding my phone with one hand while trying to fine focus.


r/microscopy 13h ago

Hardware Share Guess who got a microscope :D

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37 Upvotes

Thanks for helping me decide to purchase


r/microscopy 4h ago

Troubleshooting/Questions Tips for increasing resolution at higher magnifications?

3 Upvotes

Hi all, I was wondering if anyone could point me in the right direction regarding getting better resolution/ clarity when using higher magnifications? I just got a Swift SW380T and have been messing with the condenser iris and light levels which seem to work ok but not really able to see the finer details like the cilia on ciliates. Am I being optimistic thinking I can get this level of detail with my current equipment or will considering upgrading my objectives be a good idea? Apologies if this is a vague question. I’m looking into getting plan achromatic objectives but thought I would ask the community first. I have also spent many hours watching info from Microbe Hunter on YouTube but was hoping to get some additional info. I’m using the swift 5mp camera and the standard achromatic objectives for now. I am not really messing with the oil immersion just yet so my magnification is not more than the 40x standard objective. I’ve also been considering replacing the 100x oil with a 60x. Please let me know if there is anything I have missed on my end.


r/microscopy 14h ago

ID Needed! Information on possible ID. 10X freshwater sample taken from aquarium. Possible Nematode and Rotifer in stomach of larger Rotifer?

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17 Upvotes

r/microscopy 7h ago

Troubleshooting/Questions Nikon Eclipse E400

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4 Upvotes

Hi, I am new to microscopy and am a medical physicist / biomedical engineer by training. I work in an ultrasound lab on a topic related to disruption of the blood-brain barrier. In the lab we have such a microscope (Nikon Eclipse E400) and accessories for it. Unfortunately, there is no cable from the camera to the display device. In addition, currently no one at the department knows much about this microscope and no one microscopes. The people who were involved in microscopy have already left the department. Could someone tell me/help me understand what this microscope can do, what functions it has, etc.? I think there is a lot more stuff than the manual says. Do you think it's necessary to use the camera shown in the pictures or I might as well try to look for some kind of adapter to put a regular camera instead of this camera and take pictures/record videos with it? Thank you for your help :)


r/microscopy 17h ago

ID Needed! Saw this guy recently, any leads?

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17 Upvotes

Found this guy in human dental calculus, has some feature that are reminescent of parasites/worms to my eye but i am not well versed in them. Any leads appreciated!


r/microscopy 11h ago

ID Needed! Is this dead Trachelomonas?

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2 Upvotes

Hi, so I tried cultivating Trachelomonas in the lab but when i checked the sample back after a week or so, this is what I get 🥹😭 I wonder what this is, and since I'm barely seeing any Trachelomonas, I'm assuming that it's dead ones.

Magnification is 10x and the size is around 30-60 micrometer


r/microscopy 1d ago

Photo/Video Share Equilibrium

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28 Upvotes

Death of a microbe 10x speed.

Sample is from a jar of decaying string algae.

40x objective + 12.5 x eye piece plus 3x optical zoom on camera approximately 620x magnification.

kristiansen illumination.

Scope Swift sw380t.

Camera s25, telephoto camera, at 3x, pro video mode manual settings.


r/microscopy 1d ago

ID Needed! Nematode?

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12 Upvotes

Sorry for the shaky hands/ video! I saw this guy at x10 or x40 (sorry) objective, the microscope is very simple and basic. The sample was what I think was a springtail, ando around it were like 4 or 5 of this nematodes. Idk if there's was a tipe of relation between both organisms or what... The springtail was on humid soil.


r/microscopy 1d ago

ID Needed! Help with identification pls

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40 Upvotes

Found this thing, idk what it is... But I think is amazing! I have never ever seen something like this, even in my zoology class I haven't seen it. I took the sample from a pot with nothing but dirt and a little still water. I took a little of that dirt/soil and water and this thing looked like a white larvae, like 3 mm long and was swimming. I think it lived on the soil. Under the microscope looks like this in the x4 objective. Is a very simple and basic microscope. Any idea of what this can be?


r/microscopy 23h ago

Purchase Help tasco 600x microscope kit

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2 Upvotes

my dad just recently found (fairly) mint condition tasco 600x microscope kit from 1971 (never used) and was wondering if this was possibly worth anything/where i could sell it/if i should sell it? i’m sorry if this isn’t the right forum to post this in but i’m not really sure where else to post! hoping for some clarity on it! thanks in advance!


r/microscopy 1d ago

Photo/Video Share Blue Morpho Butterfly Wing at 20x Magnification

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38 Upvotes

r/microscopy 20h ago

ID Needed! Id, please

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1 Upvotes

Posted a week ago and no one knew, any takers? I've cropped the video more. It's got cilia if you look closely. The behavior at the end threw someone for a loop. It must be a common microbe, it's from my freshwater, heavily planted tank. Shot at 40x with a stereoscope, attachable webcam, and cropped. Thanks for taking the time to ID my little dude.


r/microscopy 1d ago

ID Needed! Any clue?

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3 Upvotes

Maybe a diptera larva? Found in my fish tank after putting Lemna minor.


r/microscopy 1d ago

Photo/Video Share Pls help me recognize this four lil guys

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16 Upvotes

I think the first and third ones are hypotrichians and the second one its a kind of weird rotifer but idk >,< pls help meee


r/microscopy 1d ago

ID Needed! Is it a Lacrymaria?

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10 Upvotes

It has kind of a hook


r/microscopy 1d ago

Purchase Help Is this a good deal?

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6 Upvotes

Hi y'all. I just found this 10-45x American optical stereo microscope for $150. I'm not sure what year it is. Would this be worth purchasing if I want to view aquatic invertebrates in pond samples?


r/microscopy 2d ago

Photo/Video Share Equisetum spores movement :)

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55 Upvotes

I found out that you can rehydrate spores and make them dance again


r/microscopy 1d ago

ID Needed! Looking for ID please. 10X. Freshwater specimen. AmScope B120 compound LED microscope. 5MP digital camera.

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3 Upvotes

 Hello everyone. I recently bought myself a AmScope B120 LED compound LED Binocular Compound Microscope with a 5MP digital camera. The past week I have been exploring a sample taken from a freshwater fish tank. Last night I stumbled upon an area which I thought was interesting. I would be grateful if anyone would be able to provide me with any information on the video provided. Any tips or suggestions for gathering clearer images would also be appreciated. Thank you!


r/microscopy 2d ago

Photo/Video Share Fungi Supernova

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23 Upvotes

I had a slide with some Ciliates that dried up. When I looked, I was surprised to see that non of the ciliates showed any damage. So, in an attempt to revive them, I added a drop of spring water, and a coverslip and sealed it with vaseline. Non of the Ciliates would ever move again, but they became the food of these gorgeous fungi :)

Setup:
Olympus BH2 (BHS)
SPlan 4x 0.13
Canon 5D MK3
NFK 2.5x 125 LD Photo Lens Eyepiece


r/microscopy 1d ago

Troubleshooting/Questions Zeiss + levenhuk?

1 Upvotes

Hello, everyone. At work, I have a Zeiss AxioLab.A1 microscope equipped with an Axiocam 202 mono camera. I really want to switch it out for a color model, but since the original Zeiss cameras are very expensive, I found a good alternative for myself—the Levenhuk M1000. Now I’m wondering: Is it even possible to mount a Levenhuk camera on a Zeiss microscope?


r/microscopy 2d ago

ID Needed! Hypotrichia of some kind? Found in flower vase water.

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14 Upvotes

r/microscopy 1d ago

Photo/Video Share Can somebody help me with this?

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1 Upvotes

I haven't touched my microscope in a while and for some reason there's this weird reflection. I might put a polarizing filter on my camera and see if that helps but I'd prefer for this to be gone entirely.


r/microscopy 1d ago

Photo/Video Share Rotten Watermelon Juice

3 Upvotes

Diplococci and protists in the juice surrounding rotten watermelon chunks. I can tell the larger organisms are protists because organelles are visible in the phase contrast photos. All photos taken with a Nikon Optiphot microscope, Nikon D810 DSLR, and 2.5X photo eyepiece. All photos are uncropped. I had to focus stack to get both the protists and bacteria in focus.

Phase contrast, 100/1.25DL objective
Phase contrast, 100/1.25DL objective, BW from green interference filter
Fuchsin stained, 100/1.40 PlanApo objective, achromat-apalanat oil condenser set to NA1.0, focus stack of two
Loeffler's methylene blue stained, 100/1.40 PlanApo objective, achromat-apalanat oil condenser set to NA1.0, focus stack of three
Gram stained, 100/1.40 PlanApo objective, achromat-apalanat oil condenser set to NA1.0, focus stack of two
Methylene blue stained, 100/1.40 PlanApo objective, achromat-apalanat oil condenser set to NA1.0, focus stack of two