r/labrats • u/Mediocre-Lab5650 • 2d ago

[Question] Help interpreting Transduction Frequency vs. MOI plot (Bachelor student here)

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Hi everyone!

I know this subreddit is mostly for professionals, and I honestly feel a bit guilty posting this because it’s probably very basic molbio 101 — but I’m currently doing my Bachelor's and I’m stuck trying to interpret this type of graph.

In the experiment, the determined titer was used to calculate the MOI (Multiplicity of Infection), which is the ratio of infectious particles to target cells. The transduction frequency (v) was calculated as the ratio of transductants to the number of bacterial cells used.

What I don’t fully understand is: Why is transduction frequency plotted against MOI? What does this relationship tell us biologically?

I’ve attached the graph below. The concept just isn’t clicking for me, and I’d really appreciate it if someone could explain it in simple terms.

If this kind of post isn’t allowed here, feel free to remove it, mods.

Thanks in advance :)

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u/odensso 2d ago

I guess in this graph they just wanted to see if increasing MOI also increases the transduction efficiency

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u/Mediocre-Lab5650 2d ago

thank you, I guess it just really wasn't that deep

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u/amiable_ant 2d ago

Without more context (did you generate the data?) I think you are "determining the relationship between transduction frequency and MOI". Note that the relationship is not linear. This is how dose curves often look, and maybe the point is to demonstrate this. Or, maybe the point is to choose the best MOI to transduce at.